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rabbit polyclonal antibody directed against cyclin d1 (ab-3  (Thermo Fisher)


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    Thermo Fisher rabbit polyclonal antibody directed against cyclin d1 (ab-3
    Rabbit Polyclonal Antibody Directed Against Cyclin D1 (Ab 3, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal antibody directed against cyclin d1 (ab-3/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    rabbit polyclonal antibody directed against cyclin d1 (ab-3 - by Bioz Stars, 2026-02
    90/100 stars

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    rabbit polyclonal antibody directed against cyclin d1 (ab-3  (Thermo Fisher)
    90
    Thermo Fisher rabbit polyclonal antibody directed against cyclin d1 (ab-3
    Rabbit Polyclonal Antibody Directed Against Cyclin D1 (Ab 3, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal antibody directed against cyclin d1 (ab-3/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    rabbit polyclonal antibody directed against cyclin d1 (ab-3 - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier
    rabbit polyclonal antibody against cyclin d1 ab 3  (Thermo Fisher)
    86
    Thermo Fisher rabbit polyclonal antibody against cyclin d1 ab 3
    Western blot analysis of cell cycle proteins from mouse epidermis. Protein lysates of epidermis samples from K5-Myc, K5-Myc/CDK4−/−, K5-Myc/CDK4+/−, CDK4−/−, CDK4+/−, and wild-type siblings were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and blotted onto a nitrocellulose membrane. Primary antibodies against CDK4, CDK6, CDK2, <t>cyclin</t> <t>D1,</t> cyclin D2, cyclin A, and cyclin E were used for immunoblot analysis. Protein levels were quantified with a densitometer.
    Rabbit Polyclonal Antibody Against Cyclin D1 Ab 3, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal antibody against cyclin d1 ab 3/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    rabbit polyclonal antibody against cyclin d1 ab 3 - by Bioz Stars, 2026-02
    86/100 stars
    		  Buy from Supplier

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    Western blot analysis of cell cycle proteins from mouse epidermis. Protein lysates of epidermis samples from K5-Myc, K5-Myc/CDK4−/−, K5-Myc/CDK4+/−, CDK4−/−, CDK4+/−, and wild-type siblings were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and blotted onto a nitrocellulose membrane. Primary antibodies against CDK4, CDK6, CDK2, cyclin D1, cyclin D2, cyclin A, and cyclin E were used for immunoblot analysis. Protein levels were quantified with a densitometer.

    Journal:

    Article Title: Lack of Cyclin-Dependent Kinase 4 Inhibits c- myc Tumorigenic Activities in Epithelial Tissues

    doi: 10.1128/MCB.24.17.7538-7547.2004

    Figure Lengend Snippet: Western blot analysis of cell cycle proteins from mouse epidermis. Protein lysates of epidermis samples from K5-Myc, K5-Myc/CDK4−/−, K5-Myc/CDK4+/−, CDK4−/−, CDK4+/−, and wild-type siblings were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and blotted onto a nitrocellulose membrane. Primary antibodies against CDK4, CDK6, CDK2, cyclin D1, cyclin D2, cyclin A, and cyclin E were used for immunoblot analysis. Protein levels were quantified with a densitometer.

    Article Snippet: The following antibodies were used: rabbit polyclonal antibodies against CDK4 (C22), CDK2 (M2), and CDK6 (C21) (all from Santa Cruz Biotechnology, Inc., Santa Cruz, Calif.); rabbit polyclonal antibody against cyclin D1 (Ab-3) (Lab Vision Corp./Neo Markers, Fremont, Calif.); and horseradish peroxidase-conjugated secondary antibody (Amersham Corp., Arlington Heights, Ill.).

    Techniques: Western Blot, Polyacrylamide Gel Electrophoresis

    CDK4 complex formation and kinase assays of K5-Myc epidermis. (A) Complex formation between cyclin D1 and CDK4. Fresh epidermal proteins from K5-Myc and wild-type mice were immunoprecipitated (IP) with a polyclonal antibody against cyclin D1 and immunoblotted with polyclonal antibodies against CDK4 and cyclin D1. Lanes A and B, protein lysates from K5-Myc and wild-type epidermis, respectively. (B) Kinase activity of CDK4 from K5-Myc transgenic and wild-type siblings. Fresh epidermal proteins from two transgenic and two wild-type mice were immunoprecipitated with an anti-CDK4 (IP CDK4) antibody, and in vitro kinase assays were carried out with a pRb peptide as a substrate. (C) Kinase activity of CDK2 from K5-Myc/CDK4+/+, K5-Myc/CDK4+/−, K5-Myc/CDK4−/−, and normal siblings. Fresh epidermal proteins were immunoprecipitated with an anti-CDK2 (IP CDK2) antibody and normal rabbit immunoglobulin G (NR), and in vitro kinase assays were carried out with histone H1 as a substrate.

    Journal:

    Article Title: Lack of Cyclin-Dependent Kinase 4 Inhibits c- myc Tumorigenic Activities in Epithelial Tissues

    doi: 10.1128/MCB.24.17.7538-7547.2004

    Figure Lengend Snippet: CDK4 complex formation and kinase assays of K5-Myc epidermis. (A) Complex formation between cyclin D1 and CDK4. Fresh epidermal proteins from K5-Myc and wild-type mice were immunoprecipitated (IP) with a polyclonal antibody against cyclin D1 and immunoblotted with polyclonal antibodies against CDK4 and cyclin D1. Lanes A and B, protein lysates from K5-Myc and wild-type epidermis, respectively. (B) Kinase activity of CDK4 from K5-Myc transgenic and wild-type siblings. Fresh epidermal proteins from two transgenic and two wild-type mice were immunoprecipitated with an anti-CDK4 (IP CDK4) antibody, and in vitro kinase assays were carried out with a pRb peptide as a substrate. (C) Kinase activity of CDK2 from K5-Myc/CDK4+/+, K5-Myc/CDK4+/−, K5-Myc/CDK4−/−, and normal siblings. Fresh epidermal proteins were immunoprecipitated with an anti-CDK2 (IP CDK2) antibody and normal rabbit immunoglobulin G (NR), and in vitro kinase assays were carried out with histone H1 as a substrate.

    Article Snippet: The following antibodies were used: rabbit polyclonal antibodies against CDK4 (C22), CDK2 (M2), and CDK6 (C21) (all from Santa Cruz Biotechnology, Inc., Santa Cruz, Calif.); rabbit polyclonal antibody against cyclin D1 (Ab-3) (Lab Vision Corp./Neo Markers, Fremont, Calif.); and horseradish peroxidase-conjugated secondary antibody (Amersham Corp., Arlington Heights, Ill.).

    Techniques: Immunoprecipitation, Activity Assay, Transgenic Assay, In Vitro